PIPETTES ESPECIALLY ADAPTED FOR USE WITH ALKALINE PYROGALLOL

Note on anaerobiosis and the use of alkaline solutions of pyrogallol.

Dye loading with patch pipettes.

This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction (e.g., Lucifer yellow); ion-sensitive indicator ...

Cleaning patch-clamp pipettes for immediate reuse

Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manu...

A New Type of Syringe, Especially Adapted for Intravenous Injections or the Aspiration of Blood

In rabbits destroying transfused blood constantly during a period of many months a pronounced and widespread siderosis ensues, practically identical with that characterizing human hemochromatosis. The findings do not indicate the ultimate cause of this disease, but they throw light on its various features and its course, and suggest a means for its diagnosis.

Metal ligand affinity pipettes and bioreactive alkaline phosphatase probes: tools for characterization of phosphorylated proteins and peptides.

An alkaline phosphatase-bioreactive probe, in which the enzyme is covalently bound to the mass spectrometry target, has been developed for studies of phosphoproteins. The bioreactive probe was used in combination with affinity capture and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to study hydrolysis of several phosphoproteins found in human saliva. Human saliv...